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3种清风藤属植物低极性部位GC-MS分析及抗炎活性

来源:用户上传      作者:崔仁杰 胡丹 邓璐璐 李江 穆淑珍

  中D分类号 R284.1 文献标志码 A 文章编号 1001-0408(2022)04-0446-06
  DOI 10.6039/j.issn.1001-0408.2022.04.11
  摘 要 目的 探究鄂西清风藤、灰背清风藤和小花清风藤低极性部位成分特点及其抗炎活性。方法 运用气相色谱-质谱技术对鄂西清风藤、灰背清风藤和小花清风藤干燥茎叶石油醚萃取液中经石油醚洗脱所得的低极性部位进行成分分析,采用NIST 17和Wiley 275数据库对色谱峰进行检索,人工比对鉴定各化合物的结构,并采用峰面积归一化法计算各成分的相对百分含量。以吲哚美辛为阳性对照,以一氧化氮(NO)生成抑制率为指标,比较上述3种清风藤属植物低极性部位对脂多糖诱导人类风湿性关节炎成纤维样滑膜细胞RA-FLS、小鼠单核巨噬细胞RAW264.7生成NO的影响,并计算半数抑制浓度(IC50)。结果 从鄂西清风藤、灰背清风藤和小花清风藤低极性部位中分别确认了70、40、33种成分,均包含长链脂肪烃、长链醇/醛/酯、单萜等成分,但三者共有成分仅有正十二烷、正二十二烷等5种。鄂西清风藤中萜类成分丰富(40.89%),灰背清风藤中三萜成分的相对百分含量较高(30.29%),小花清风藤中长链脂肪烃成分的相对百分含量较高(87.70%)。上述3种清风藤属植物低极性部位对RA-FLS细胞NO生成的IC50分别为2.22、1.69、4.15 μg/mL,对RAW264.7细胞NO生成的IC50分别为1.23、4.02、3.05 μg/mL,均低于吲哚美辛(12.97、10.66 μg/mL)。结论 上述3种清风藤属植物低极性部位均含有长链脂肪烃、单萜、长链醇/醛/酯等成分,且三者均具有一定的抗炎活性。
  关键词 清风藤属;鄂西清风藤;灰背清风藤;小花清风藤;低极性部位;气相色谱-质谱技术;成分分析;抗炎活性
  GC-MS analysis and anti-inflammatory activity of low polarity parts from 3 species of Sabia genus
  CUI Renjie1,2,HU Dan3,DENG Lulu2,3,LI Jiang2,3,MU Shuzhen2,3(1. College of Pharmacy, Guizhou University of Traditional Chinese Medicine, Guiyang 550025, China; 2. Key Laboratory of Chemistry for Natural Products of Guizhou Province and Chinese Academy of Sciences, Guiyang 550014, China; 3. State Key Laboratory of Functions and Applications of Medicinal Plants, Guizhou Medical University, Guiyang 550014, China)
  ABSTRACT OBJECTIVE To investigate the component characteristics and anti-inflammatory activities of the low polarity parts from Sabia campanulata, S. discolor and S. parviflora. METHODS Gas chromatography-mass spectrometry was used to analyze the components of low polarity parts eluted by petroleum ether from petroleum ether extract of dried stems and leaves of S. campanulata, S. discolor and S. parviflora. The chromatographic peaks were retrieved by both NIST 17 and Wiley 275 database, and their structures of each compound were compared and identified manually. The relative percentage content of each component was calculated by peak area normalization method. With indomethacin as positive control and the inhibition rate of nitric oxide (NO) production as the index, the effects of the low polarity parts of the above three species of Sabia genus on the production of NO in lipopolysaccharide-induced human rheumatoid arthritis fibroblast-like synoviocytes (RA-FLS) and mouse mononuclear macrophage RAW264.7 were compared; the half inhibitory concentration (IC50) was calculated. RESULTS Totally 70, 40 and 33 chemical components were identified respectively from the low polarity parts of S. campanulata, S. discolor and S. parviflora, including long-chain fatty hydrocarbons, long-chain alcohol/aldehyde/ester, monoterpenes; but there were only 5 common components in the three species, such as dodecane and docosane, etc. S. campanulata contained abundant terpenoids (40.89%); the relative percentage content of triterpenoids was high in S. discolor (30.29%); the relative percentage content of long-chain fatty hydrocarbons was high in S. parviflora (87.70%). The values of IC50 of the low polarity parts of the above three species of Sabia genus to the production of NO in RA-FLS cells were 2.22, 1.69 and 4.15 μg/mL, respectively. The values of IC50 of them to the production of NO in RAW264.7 cells were 1.23, 4.02 and 3.05 μg/mL respectively, and all of them were lower than indomethacin (12.97, 10.66 μg/mL). CONCLUSIONS The components of the low polarity parts from the above three species of Sabia genus are mainly long-chain fatty hydrocarbons, monoterpenes and long-chain alcohol/aldehyde/ester, and all of them have certain anti-inflammatory activities.
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