甜玉米维生素E含量变异及杂种优势分析

来源:用户上传      作者:谢利华 胡建广 李春艳 肖颖妮 李武 于永涛 卢文佳 文天祥 李高科

　　摘要：【目的】分析不同甜玉米s交种的杂种优势，筛选高维生素E（VE）组合及优异亲本，为高效创制高VE甜玉米品种提供材料基础和理论依据。【方法】采用正相高效液相色谱法（NP-HPLC），对11份不同来源且表型差异明显的甜玉米自交系及其11个杂交种进行VE组分含量测定，并分析其杂种优势，筛选高VE甜玉米材料。【结果】甜玉米自交系及杂交种中的VE组分间含量差异均达极显著水平（P<0.01，下同）。自交系26F白、金皇和杂交种粤甜41号、粤甜29号、粤甜35号的总生育酚（TTF）、总生育三烯酚（TT3）和总VE（TVE）含量较高。杂交种VE组分含量与双亲均值和高值亲本相关，其中α-生育酚（αT）和α-生育三烯酚（αT3）含量与高值亲本和双亲均值呈显著（P<0.05）或极显著正相关。11个杂交种VE组分含量的杂种优势主要表现为负向中亲优势，TVE含量的中亲优势变幅为-39.07%～56.97%，超亲优势变幅为-52.44～49.28%。11个杂交种中有4个表现出超亲优势，其中TY6和农宝2021白组配的杂交种粤甜41号、粤甜38号和粤甜35号具有较强的超亲优势，且TVE含量较高。【结论】不同基因型甜玉米间VE组分含量存在明显差异，VE含量的杂种优势主要表现为负向中亲优势;高VE亲本间杂交并不一定能够获得具有强杂种优势的杂交种。要提高甜玉米杂交种VE含量，选择高VE含量尤其是高αT和αT3含量的材料进行组配或许可加速品种培育进程。
　　关键词： 甜玉米;自交系;维生素E;杂种优势
　　中图分类号： S513 文献标志码： A 文章编号：2095-1191（2021）12-3350-08
　　Analysis of vitamin E content and heterosis in sweet
　　corn（Zea mays L.）
　　XIE Li-hua， HU Jian-guang， LI Chun-yan， XIAO Ying-ni， LI Wu， YU Yong-tao，
　　LU Wen-jia， WEN Tian-xiang， LI Gao-ke*
　　（Crops Research Institute， Guangdong Academy of Agricultural Sciences/Guangdong Provincial Key Laboratory
　　of Crop Genetic Improvement， Guangzhou 510640， China）
　　Abstract：【Objective】The heterosis of hybrids derived from different sweet corn inbred lines were analyzed to excavate the combination and parents with high vitamin E（VE） content，so as to provide materials and theoretical basis for efficiently breeding high VE content sweet corn varieties. 【Method】Normal phase high performance liquid chromatography （NP-HPLC） was used to determine the content of VE components in 11 sweet corn inbred lines with different origins and obvious phenotypic differences and their 11 hybrid combinations. The heterosis of VE components was analyzed to screen the high content sweet corn materials. 【Result】The contents of VE components in inbred lines and hybrids indicated extremely significance level（P<0.01， the same below）. The contents of total tocophenol （TTF），total tocophenol （TT3） and total VE（TVE） in inbred lines 26F Bai，Jinhuang and hybrids Yuetan 41， Yuetan 29 and Yuetan 35 were high. The contents of all VE components in hybrids were correlated with the mean values of parents and high-value parents;of which the contents of α-tocopherol （αT） and α-tocoptrienol （αT3） were significantly（P<0.05） or extremely positively correlated with the mean values of parents and high-value parents. In terms of heterosis analysis， the heterosis of VE content in 11 hybrids mainly showed negative heterosis; the TVE content mid-parentheterosisranged from -39.07% to 56.97%，and the over-parent heterosis ranged from -52.44 to 49.28%. Four hybrids showed super-parent heterosis，and the hybrids Yuetian 41，Yuetian 38 and Yuetian 35 which derived from inbred lines TY6 and Nongbao 2021 Bai presented stronger heterosis and higher TVE content. 【Conclusion】There are differences in VE content among different genotypes of sweet corn. The heterosis of VE content is mainly negative mid-parent heterosis. The parents with high VE contents may not obtain hybrid with strong heterosis. For improving the VE content in sweet corn，the materials with high VE content，especially those with high αT or αT3 contents，can be selected as parents to accelerate the breeding process.

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