基于网络药理学分析枸杞治疗阿尔茨海默病的作用机制

来源:用户上传      作者:彭罡 郭志芬 严大鹏 周妍 张孟俊 裘齐宁

　　[摘要]目的采用网络药理学分析枸杞治疗阿尔茨海默病的作用机制。方法初步筛选枸杞的主要成分及作用靶点（数据来源于 BATMAN-TCM 和 TCMID 数据库），收集阿尔茨海默病疾病靶点（数据来源于GeneCards和Drugbank数据库）;取枸杞与阿尔茨海默病交集靶点作为研究对象，构建枸杞活性成分-作用靶点-疾病的相互作用（Cytoscape）。使用 David 数据库对关键靶点进行 GO 功能富集和 KEGG 通路分析。Y果枸杞中筛选出35种主要的有效成分，其与阿尔茨海默病的交集靶点一共54个，通过 GO 富集和 KEGG 富集显示，这些靶基因主要位于突触后膜，生物过程主要涉及氧化还原过程，分子功能主要涉及黄素腺嘌呤二核苷酸合成、NADP 结合及氧化还原酶的活性。主要的信号通路涉及氨基酸的生物合成与代谢信号通路。结论枸杞治疗阿尔茨海默病具有多成分、多靶点的特点，其发挥作用的机制不仅通过在脑内发挥抗氧化的作用，而且还与氨基酸的生物合成与代谢信号通路有关。
　　[关键词]枸杞;阿尔茨海默病;抗氧化;氨基酸生物合成与代谢;网络药理学
　　[中图分类号] R285.5 [文献标识码] A [文章编号]2095-0616（2022）09-0004-04
　　Network pharmacology-based analysis on the mechanism of Lyciumbarbarum in the treatment of Alzheimer's disease
　　PENG Gang1GUO Zhifen（ 2） YAN Dapeng1 ZHOU Yan1 ZHANG Mengjun QIU Qining1
　　1. Department of Pharmacy， Zhongshan Hospital， Fudan University （Xiamen Branch）， Fujian， Xiamen 361015， China;2. Department of Pharmacy， Xiamen Humanity Hospital， Fujian， Xiamen 361015， China
　　[Abstract] Objective To analyze the mechanism of Lyciumbarbarum in the treatment of Alzheimer's disease using network pharmacology. Methods The main components and action targets of Lyciumbarbarum （sourced from BATMAN-TCM and TCMID databases） were preliminarily screened， and Alzheimer's disease targets （sourced from GeneCards and Drugbank databases） were collected. The intersection targets of Lyciumbarbarum and Alzheimer's disease were taken as study subjects， and the network map of "active components of Lyciumbarbarum - target - disease" was constructed using Cytoscape. GO functional enrichment analysis and KEGG pathway enrichment analysis were performed on the key targets using David database. Results A total of 35 major effective components in Lyciumbarbarum were screened， and a total of 54 intersection targets between effective components and Alzheimer's disease were identified. The GO enrichment analysis and KEGG enrichment analysis showed that these target genes were mainly located in the postsynaptic membrane， the biological processes were mainly involved in redox processes， and the molecular functions were mainly involved in the synthesis of flavin adenine dinucleotide， NADP binding and oxidoreductase activity. The main signaling pathways involved amino acid biosynthesis and metabolism. Conclusion Lyciumbarbarum demonstrates multi-component and multi-target characteristics in the treatment of Alzheimer's disease， and the exertion of its mechanism is not only through the antioxidant effect in the brain， but also related to the signaling pathways of amino acid biosynthesis and metabolism.

nlc202207271612

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