基于多元统计分析的山银花抗氧化与抑菌质量标志物预测

来源:用户上传      作者:王志辉　周新茹　成惠珍　王智　童巧珍　周日宝　刘湘丹

　　 〔摘要〕 目的 建立灰置忍冬抗氧化和抑菌谱-效关系质量评价系统，初步筛选出与抗氧化和抑菌药效相关的质量标志物。方法 收集湖南各产地灰毡毛忍冬样品共20份，采用HPLC建立指纹图谱，并测定各样品的抗氧化和抑菌效果，通过SPSS 23.0软件对药效指标和共有峰面积进行偏最小二乘法回归、灰色关联度和相关性分析。结果 关联度法分析得出3、12号峰与抗氧化和抑菌均有很强的相关性，偏最小二乘法回归系数可知2、3号峰与抗氧化效果相关性强，2、3、5、7、9、13、17、18号峰成分与抑菌效果相关性强，由双变量相关性分析可知3号峰与抗氧化效果显著相关，4、11和12号峰与抑菌效果显著相关，经对照品指认3号峰为绿原酸，7号峰为咖啡酸，11号峰为异绿原酸B，12号峰为异绿原酸A，17号峰为灰毡毛忍冬皂苷甲，18号峰为川续断皂苷乙，2、5、9、13号峰成分有待进一步确认。结论 多种分析方法分析了山银花抗氧化和抑菌的“谱-效”和“量-效”关系，结果一致，预测有机酸类为抗氧化药效的质量标志物，皂苷和有机酸类为抑菌的质量标志物。
　　 〔关键词〕 灰毡毛忍冬;抗氧化;抑菌;谱-效关系;量-效关系;质量标志物
　　 〔中图分类号〕R284.1 〔文献标志码〕A 〔文章编号〕doi：10.3969/j.issn.1674-070X.2022.07.009
　　Prediction of antioxidant and antibacterial quality markers of Flos lonicerae
　　based on multivariate statistical analysis
　　WANG Zhihui ZHOU Xinru CHENG Huizhen WANG Zhi TONG Qiaozhen ZHOU Ribao LIU Xiangdan
　　（1. College of Pharmacy， Hunan University of Chinese Medicine， Changsha， Hunan 410208， China; 2. Key Research Office of Germplasm Resources and Standardized Planting of Medicinal Materials of Hunan Province， Changsha， Hunan 410208， China;
　　3. Key Laboratory of Modern Research of TCM， Education Department of Hunan Province， Changsha， Hunan 410208， China）
　　〔Abstract〕 Objective To establish a quality evaluation system for the antioxidant and antibacterial spectrum-effect relationship of lonicera macranthoides， and preliminarily screen the quality markers related to antioxidant and antibacterial. Methods A total of 20 samples of lonicera macranthoides from various places in Hunan were collected. HPLC fingerprints were established， and the antioxidant and antibacterial effects of each sample were determined. The pharmacodynamic indexes and common peak areas were analyzed by partial least square regression， grey correlation and correlation analysis using SPSS software. Results The correlation degree analysis showed that peaks 3 and 12 were strongly correlated with antioxidant and antibacterial. The regression coefficient of partial least square regression showed that peaks 2 and 3 were strongly correlated with antioxidant effect， and the components of peaks 2， 3， 5， 7， 9， 13， 17 and 18 were strongly correlated with antibacterial effect. The bivariate correlation analysis showed that peak 3 was significantly correlated with antioxidant effect， and peaks 4， 11 and 12 were significantly correlated with antibacterial effect. According to the reference substance， peak 3 was chlorogenic acid， peak 7 was caffeic acid， peak 11 was isochlorogenic acid B， peak 12 was isochlorogenic acid A， peak 17 was lonicera macranthoides saponin A， peak 18 was dipsacus saponin B， and the components of peaks 2， 5， 9 and 13 needed to be further confirmed. Conclusion The "spectrum-effect" and "dose-effect" relationships between antioxidant and antibacterial of Flos lonicerae are analyzed by various analytical methods. The results are consistent and mutually confirmed. It is predicted that organic acids are the quality markers of antioxidant， saponins and organic acids are the quality markers of antibacterial.

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