猪苓多糖对LPS诱导的急性肺损伤模型大鼠炎症因子的影响

来源:用户上传      作者:李全有 刘海军

　　【摘 要】 目的：探究i苓多糖对LPS诱导的急性肺损伤模型大鼠炎症因子的影响。方法：雄性SD大鼠，腹腔注射LPS（5 mg/kg）构建急性肺损伤模型，用猪苓多糖（250 mg/kg）灌胃进行处理。计算肺组织湿干重比，HE染色检测肺组织病理学变化，ELISA检测血清中转化生长因子（TGF）-α、白介素-6（IL-6）、白介素-10（IL-10）和白介素-1β的水平，肺组织中丙二醛（MDA）、谷胱甘肽过氧化酶（GSH-Px）、髓过氧化物酶（MPO） 和超氧化物歧化酶（SOD）的表达水平。结果：Model组肺组织损伤严重，而猪苓多糖治疗能够有一定程度缓解肺损伤;与Control组相比，Model组肺组织湿干重比，TGF-α、IL-18、IL-6和IL-1β表达，以及MDA和MPO表达水平升高，而GSH-Px和SOD表达水平明显降低，差异均具有统计学意义（P<0.05）;与Model组相比，猪苓多糖组肺组织湿干重比，TGF-α、IL-18、IL-6和IL-1β表达水平，以及MDA和MPO表达水平有所降低，而GSH-Px和SOD表达水平升高，差异均具有统计学意义（P<0.05）。结论：猪苓多糖可以缓解LPS诱导的急性肺损伤模型大鼠炎症反应。
　　【关键词】 猪苓多糖;急性肺损伤模型大鼠;TGF-α;IL-1β;IL-18
　　【中图分类号】R563.9 【文献标志码】 A 【文章编号】1007-8517（2021）22-0021-04
　　Effects of Polyporus Umbellatus Polysaccharide on Inflammatory Factors in Rats with LPS-induced Acute Lung Injury
　　LI Quanyou LIU Haijun
　　Department of pharmacy， Anyang Hospital of traditional Chinese medicine，Henan Province，Anyang 455000，China
　　Abstract：Objective To explore the effects of Polyporus umbellatus polysaccharide on inflammatory factors in rats with acute lung injury induced by LPS. Methods Male SD rats were purchased， LPS （5 mg/kg） was injected intraperitoneally to construct an acute lung injury model， and polyporus polysaccharide （250 mg/kg） was administered by gavage. Calculate the wet-to-dry weight ratio of lung tissue， HE staining to detect lung tissue pathological changes， ELISA to detect transforming growth factor（TGF）-α， interleukin-6（IL-6）， interleukin-10（IL-10） and interleukin- 1β level， the expression level of malondialdehyde（MDA）， glutathione peroxidase（GSH-Px）， myeloperoxidase （MPO） and superoxide dismutase （SOD） in lung tissue.Results The lung tissue damage in the Model group was severe， and Polyporus umbellatus polysaccharide treatment could relieve the lung damage to a certain extent; compared with the Control group， the lung tissue wet-to-dry weight ratio， TGF-α， IL-18， IL-6 and IL in the Model group -1β expression， as well as the expression levels of MDA and MPO increased， while the expression levels of GSH-Px and SOD were significantly reduced（P<0.05）; compared with the Model group， the lung tissue wet-to-dry weight ratio of the polyporus polysaccharide group， TGF-α， The expression levels of IL-18， IL-6 and IL-1β， as well as the expression levels of MDA and MPO decreased， while the expression levels of GSH-Px and SOD increased （P<0.05）.Conclusion Polyporus umbellatus polysaccharide can alleviate the inflammatory response in rats with acute lung injury induced by LPS.
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