象皮生肌膏对糖尿病皮肤溃疡修复的效果及其机理探究

来源:用户上传      作者:雷宇　周忠志　兰宏伟　王婷婷　黄新灵

　　[摘要]目的：研究象皮生肌膏对糖尿病模型大鼠皮肤溃疡的修复作用，初步探究象皮生肌膏的作用机理。方法：选取80只清洁级雄性SD大鼠，随机分成4组，正常组、模型组、贝复新组、象皮生肌膏组。各组分别在治疗7、14 d后观察各组皮肤溃疡的修复状况、检测皮肤溃疡组织中IL?6和TNF?α的表达情况。结果：象皮生肌膏组和贝复新组大鼠溃疡创面的修复率较模型组显著增加，差异有统计学意义（P<0.01），而且象皮生肌膏组修复率较贝复新组更高，差异有统计学意义（P<0.05）。与正常组相比，模型组内IL-6、TNF-α的含量显著增加（P<0.01）。与模型组相比，象皮生肌膏组及贝复新组创面组织匀浆中的IL-6、TNF-α的含量显著下降（P<0.01），且象皮生肌膏组较贝复新组各指标含量下降更明显（P<0.05）。结论：象皮生肌膏可以加快糖尿病皮肤溃疡的修复，其作用机理可能是调控创面炎性因子IL-6、TNF-α的含量，减少炎性细胞的聚集，加快皮肤溃疡创面的修复。
　　[关键词]象皮生肌膏;糖尿病;皮肤溃疡;炎症因子;白细胞介素-6;肿瘤坏死因子-α
　　[中D分类号]R587.2 [文献标志码]A [文章编号]1008-6455（2022）01-0089-03
　　Effect of Xiangpi Shengji Ointment on IL-6 and TNF-α in Diabetic Skin Ulcer
　　LEI Yu1，2，ZHOU Zhongzhi1，LAN Hongwei1，WANG Tingting1，HUANG Xinling1
　　（1.Hunan University of Chinese Medicine，Changsha 410208，Hunan，China;2.College of Traditional Chinese Medicine，Changsha Medical University，Changsha 410219，Hunan，China）
　　Abstract： Objective To observe the effect of Xiangpi Shengji ointment on promoting the healing of diabetic skin ulcer and to study its effect on the inflammatory factors IL-6 and TNF-α in diabetic skin ulcer. Methods Selected 80 clean male SD rats，they were randomly divided into 4 groups：normal group， model group， Berfuxin group and Xiangpi Shengji ointment group. The repair status of skin ulcer and the expression of IL-6 and TNF-α in skin ulcer tissue were observed at 7 and 14 days after intervention. Results The repair rate of ulcer wound in Xiangpi Shengji ointment group and beifuxin group was significantly higher than that in model group （P<0.01）， and the repair rate in Xiangpi Shengji ointment group was higher than that in beifuxin group （P<0.05）. Compared with the normal group， IL-6 and TNF in the model group- α The content of was significantly increased （P<0.01）. Compared with the model group， IL-6 and TNF in the wound tissue homogenate of Xiangpi Shengji ointment group and beifuxin group- α The content of all indexes in Xiangpi Shengji ointment group decreased significantly （P<0.01）， and the content of all indexes in Xiangpi Shengji ointment group decreased more significantly than that in beifuxin group （P<0.05）. Conclusion Xiangpi Shengji ointment can accelerate the repair of diabetic skin ulcer. Its mechanism may be to regulate the content of IL-6 and TNF-α， reduce the aggregation of inflammatory cells， and promote the repair of skin ulcer.
　　Key words： xiangpi shengji ointment; diabetes; skin ulcer; flammatory cytokines; interleukin-6; tumor necrosis factor-α
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