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LncRNA HOTTIP在胃癌D2根治术后肿瘤复发、耐药及预后中的作用

来源:用户上传      作者:沈珠花 徐永灿 乔鹏

  [摘要] 目的 分析LncRNA HOTTIP在 胃癌D2根治g后肿瘤复发、耐药及预后中的作用。 方法 随机选取2018年1月至2020年6月在湖州市中心医院行胃癌D2根治术的126例胃癌患者作为研究对象。 结果 胃癌组织中LncRNA HOTTIP表达水平明显升高,胃癌组织LncRNA HOTTIP表达水平与TNM分期、淋巴结转移、侵袭深度及术后复发明显相关。TNM分期、侵袭深度越高并伴有淋巴结转移及术后复发的患者LncRNA HOTTIP越高。Kaplan-Meier生存曲线分析,LncRNA HOTTIP高表达组患者生存期均明显低于LncRNA HOTTIP低表达组患者。SGC7901/DDP组细胞LncRNA HOTTIP表达水平明显高于SGC7901组(P<0.01)。LncRNA HOTTIP低表达组细胞增殖能力及凋亡能力明显强于对照组,存活率明显低于对照组(P<0.05)。 结论 LncRNA HOTTIP在胃癌组织中的表达水平明显升高,且其表达水平与患者的复发及预后明显相关;经实验发现,下调LncRNA HOTTIP的表达能够明显抑制细胞增殖,抑制细胞活性,促进细胞凋亡,降低胃癌细胞的耐药性,提高对化疗药物的敏感性。
  [关键词] LncRNA HOTTIP;胃癌D2根治术;肿瘤复发;耐药;预后
  [中图分类号] R735.2 [文献标识码] B [文章编号] 1673-9701(2022)13-0042-05
  [Abstract] Objective To analyze the role of LncRNA HOTTIP in tumor relapse, drug resistance and prognosis after D2 radical surgery for gastric cancer. Methods A total of 126 patients with gastric cancer who underwent D2 radical surgery for gastric cancer in Huzhou Central Hospital from January 2018 to June 2020 were randomly selected as study subjects. Results The expression level of LncRNA HOTTIP was significantly increased in gastric cancer tissues,which was significantly correlated with tumor node metastasis (TNM) stage,lymph node metastasis,invasion depth and postoperative relapse.The higher the TNM stage and the deeper the invasion depth, the higher the LncRNA HOTTIP expression level in patients with lymph node metastasis and postoperative relapse.The Kaplan-Meier survival analysis showed that the survival period was shorter in patients with high-expression level of LncRNA HOTTIP than that in patients with low-expression level of LncRNA HOTTIP.The expression level of LncRNA HOTTIP in the SGC7901/DDP group was significantly higher than that in the SGC7901 group(P<0.01).The cell proliferation ability and apoptosis ability in the group with low-expression level of LncRNA HOTTIP were significantly higher than those in the control group, and the survival rate was significantly lower than that in the control group(P<0.05). Conclusion The expression level of LncRNA HOTTIP is significantly increased in gastric cancer tissues, and its expression level is significantly correlated with the tumor relapse and prognosis of patients. Meanwhile, it is found that the down-regulation of LncRNA HOTTIP expression can significantly inhibit cell proliferation,suppress cell activity,promote apoptosis, reduce drug resistance of gastric cancer cells,and improve the sensitivity to chemotherapeutic drugs.

nlc202206161036



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